IIT Hyderabad Junior Technician- Biomedical Engineering Exam Syllabus

IIT Hyderabad Junior Technician- Biomedical Engineering Exam Syllabus 2026

Syllabus for Preliminary and Main Written Test.

1. Laboratory Safety & Compliance
   a. Good Laboratory Practices (GLP) and standard operating procedures (SOPs)
   b. Biosafety levels (BSL-1 and BSL-2)- containment, access control, and PPE
   c. Biological, chemical, and sharp waste segregation and disposal
   d. Spill management and emergency procedures
   e. Awareness of NABL / GLP / ISO concepts (introductory level)
2. Basic Laboratory Techniques
   a. Preparation of reagents, buffers, and media (molarity, normality, pH)
   b. Sterilization methods- autoclaving, filtration, dry heat, chemical sterilants
   c. Calibration and routine maintenance of laboratory instruments
   d. Record keeping, labeling, and sample traceability
3. Cell Biology (Fundamentals)
   a. Structure and function of prokaryotic and eukaryotic cells
   b. Cell cycle, apoptosis, necrosis
   c. Basics of cell signaling and differentiation
   d. Overview of stem cells (introductory level)
4. Mammalian Cell Culture
   a. Aseptic techniques and laminar airflow operation
   b. Types of cell cultures- primary, secondary, continuous cell lines
   c. Culture media, supplements, sera, and antibiotics
   d. Thawing, passaging, sub-culturing, and cryopreservation
   e. Cell counting and viability assessment
   f. Identification, prevention, and management of contamination (fungal, bacterial, mycoplasma)
   g. Basics of 2D vs 3D culture systems (awareness level)
5. Core Biochemical Techniques
   a. Pipettes and pipetting accuracy
   b. Spectrophotometry- Beer–Lambert law and applications
   c. Protein estimation (Bradford, Lowry, BCA – principles)
   d. Agarose gel electrophoresis
   e. SDS-PAGE and Western blotting (workflow and purpose)
   f. ELISA- principle, types, and applications
6. Basic Molecular Biology
   a. DNA/RNA isolation – principles
   b. PCR and RT-PCR- components and applications
   c. Storage and handling of biological samples
7. Animal Facility Management
   a. Laboratory animals commonly used in research (rats, mice, rabbits)
   b. Animal housing, feeding, and environmental conditions
   c. Health monitoring and record keeping
8. Animal Handling & Experimental Procedures
   a. Handling and restraint techniques
   b. Routes of drug administration
   c. Blood collection methods
   d. Tissue collection and sample processing
   e. Basic anesthesia and euthanasia methods (ethical aspects)
9. Regulatory & Ethical Framework
   a. CPCSEA guidelines and institutional animal ethics committees (IAEC)
   b. 3Rs principle (Replacement, Reduction, Refinement)
   c. Ethics in animal experimentation and humane endpoints
10. Computer proficiency
    a. Fundamentals of computer operation and file management
    b. Use of MS Word or similar for reports, SOPs, and documentation
    c. Use of MS Excel or similar for data entry, basic formulas, and inventory records
    d. Email communication and handling of attachments
    Skill Test / Practical Assessment
    a. Demonstration of aseptic technique
    b. Proper pipetting and solution preparation
    c. Cell culture handling (mock or live demonstration)
    d. Awareness of animal handling ethics and procedures
    e. Instrument handling and safety compliance