| S. No. |
Topic |
| 1 |
Growing of bacterial culture in solid media using the spread plate and streaking method. |
| 2. |
Estimation of Protein content in the given sample by Lowry’s method |
| 3. |
Estimation of reducing sugar in the given sample by dinitro-salicylic (DNS) method |
| 4. |
Extraction of amylase from the given sample |
| 5. |
Determination of COD (chemical oxygen demand) of given waste water sample |
| 6. |
Preparation and running of Agarose gel electrophoresis of the given DNA sample and the recovery of the DNA from the gel. |
| 7. |
Restriction Digestion of DNA using restriction enzymes |
| 8. |
Release of the intracellular products/ components from the given microbial cells into the solution by sonication; determination of enzyme activity and protein content of the intracellular products |
| 9 |
Determination of the blood group of an individual |
| 10 |
Immobilization of enzyme using calcium alginate beads and determination of enzyme activity. |
| 11 |
Retrieval of gene/ protein sequences from biological databases, multiple sequence alignment and construction of the phylogenetic free. |
| 12. |
Basic knowledge of Microsoft office tools like word, excel and power point. Data analysis in excel and plotting different kinds of graphs like scatter plot, bar and pie chart etc. |
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