NIT Jalandhar Technical Assistant/Technician Biotechnology Syllabus for Paper III
NIT Jalandhar Technical Assistant/ Technician Biotechnology stage III (Final written Test) shall be of two hours duration comprising of 100 multiple choice questions. Each correct answer will be awarded one mark and for each wrong answer one fourth (1/4) mark shall be deducted. Only those who are screened in after the screening test (stage I) and qualify the skill test (stage II) will be allowed to appear in the final test (stage III).
| Biochemistry | Bio molecules-structure and functions; Biological membranes, structure, action potential and transport processes; Enzymes- classification, kinetics and mechanism of action; Basic concepts of metabolism (carbohydrates, lipids, amino acids and nucleic acids) photosynthesis, respiration and electron transport chain; Bioenergetics. |
| Microbiology | Viruses – structure and classification; Microbial classification and diversity (bacterial, algal and fungal); Methods in microbiology; Microbial growth and nutrition; Aerobic and anaerobic respiration; Nitrogen fixation; Microbial diseases. |
| Molecular Biology and Genetics | Molecular structure of genes and chromosomes; Mutations and mutagenesis; Nucleic acid replication, transcription, translation and their regulatory mechanisms in prokaryotes and eukaryotes; Microbial genetics (plasmids, transformation, transduction, conjugation) |
| Analytical Techniques | Principles of microscopy-light, electron, fluorescent and confocal; Centrifugation – high speed and ultra; Principles of spectroscopy – UV, visible; Principles of chromatography – ion exchange, gel filtration, hydrophobic interaction, affinity, GC, HPLC, FPL; Electrophoresis |
| Recombinant DNA Technology | Restriction and modification enzymes; Vectors; plasmid, bacteriophage and other vectors, cosmids, Ti plasmid vectors; cDNA and genomic DNA library; Gene isolation, cloning and expression; DNA sequencing; Polymerase chain reactions; Southern and Northern blotting; In-situ hybridization; Gene transfer technologies. |
| Bioprocess Engineering and Process Biotechnology | Chemical engineering principles applied to biological system; Principle of reactor design, multiphase bioreactors, mass and heat transfer; Rheology of fermentation fluids, Aeration and agitation; Media formulation and optimization; Kinetics of microbial growth, substrate utilization and product formation; Sterilization of air and media, Batch, fed-batch and continuous processes; Various types of microbial and enzyme reactors; Unit operations in solid- liquid separation and liquid – liquid extraction, Process scale up.
Production of biomass and primary/ secondary metabolites; Biofuels, industrial enzymes, antibiotics; Large scale production and purification of recombinant proteins; Industrial application of membrane based bio separation methods; Immobilization of biocatalysts (enzymes and cells) for bioconversion processes; Bioremediation- aerobic and anaerobic processes for stabilization of solid/ liquid wastes. |
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