MPPSC Scientific Officer Biochemistry Paper 2013

MPPSC Scientific Officer Biochemistry Paper 2013

Industrial production of acetic acid uses
(a) Green algae
(b) Cyanobacteria
(c) Aerobic bacteria
(d) Anaerobic bacteria

Agrobacterium is very useful in
(a) Plant genetic engineering
(b) Nitrogen fixation
(c) Alcohol fermentation
(d) Antibiotic production

Immobilized enzymes mean
(a) potentiatin of activity
(b) Presentation of activity
(c) Preparation of activity
(d) All of these

A particular RNA in a mixture can be identified by
(a) Western blotting
(b) Eastern blotting
(c) Northern blotting
(d) Southern blotting

Transgenic animals may be prepared by introducing a foreign gene into
(a) Somatic cells of yound animals
(b) Tests and ovaries of animals
(c) A viral vector and infecting the animals with the viral vector
(d) Fertilised egg and implanting the egg into a foster mother

Lambda phage can be used to clone DNA fragments of the size
(a) upto 3 kilobases
(b) upto 20 kilobases
(c) upto 45 kilobases
(d) upto 1,000 kilobases

In Sanger’s method of DNA sequence determination, DNA synthesis is stopped by using
(a) 1′,2′- dideoxyribonucleoside triphosphates
(b) 2′,3′- Dideoxyribonucleoside triphosphates
(c) 2′, 4′ – Diedeoxyribonucleoside triphosphates
(d) 2′, 5′- Dideoxyribonucleoside triphosphates

Binding of RNA polymerase holoenzyme to the promoter region of lac operon is facilitated by
(a) Catabolite gene activator protein (CAP)
(b) CAMP
(c) CAP – CAMP complex
(d) None of the above

Reverse transcriptase activity is present in the eukaryotic
(a) DNA polymerase Alpha
(b) Helicase
(c) Telomerase
(d) DNA polymerase II

Restriction endonucleases
(a) Cut RNA chains at specific locations
(b) Excise introns from mRNA
(c) Act as defensive enzymes to protect the host bacterial DNA from DNA of foreign organisms
(d) Remove Okazaki fragments

Repressor binds to DNA sequence and regulate the transcription. This sequence is called
(a) Attenuator
(b) Terminator
(c) Anti-terminator
(d) Operator

jIn biosynthesis of proteins, the chain terminating codons are
(a) UGG, UGU and AUG
(b) UAA, UAG and UGA
(c) AAU, AAG and GUA
(d) UAA, AUG and UAG

Genetic code was unravelled by
(a) Ochoa – Niranberg
(b) Crick – Watson
(c) Beadle – Tatum
(d) Sanger – Perutz

During replication, the template DNA is unwound
(a) At one of the ends
(b) At both the ends
(c) At multiple sites
(d) Nowhere